The top one is Crona's sex so it's what he biologically is. Gender is what YOU process as. Crona has never clarified for us so yes it is unknown. Either way Crona is still my favorite. Right above Kid.
Roymaes: “The boy who can perform alchemy without a transmutation circle… Edward Elric… the Fullmetal Alchemist!”>>>> he goes from a terrified little kid who barely knows why he's doing, to a confident, mature adult. I will love this manga forever